pi cell cycle analysis kit  (Canvax Inc)

Journal: RSC Advances

Article Title: β-caryolane derivatives as novel anti-colorectal cancer agents: synthesis and in vitro biological evaluation

doi: 10.1039/d5ra07312j

Figure Lengend Snippet: NC-19 induces HT-29 G0/G1 phase cell cycle arrest and ROS generation. (A and B) Cell cycle analysis of HT-29 cell cycle distribution after treatment with NC-19 (0, 2, 4, 8 µM) for 24 hours. (C and D) Western blot and bar graph analysis of CDK2 and Cyclin E protein expression in HT-29 cells after treatment with NC-19 (0, 5, 10, 20 µM) for 24 hours. (E) Cell viability of HT-29 cells pretreated with NAC (2 mM) for 8 hours, followed by incubation with or without 2 µM NC-19 for 72 hours. (F) Detection of ROS generation by DCFH-DA in HT-29 cells after treatment with NC-19 (0, 1, 2, 5, 10 µM) for 24 hours.

Article Snippet: NAC, DCFH-DA, RIPA lysis buffer, protease inhibitors, phosphatase inhibitors, 4% paraformaldehyde fix solution, hematoxylin and eosin staining kit, Western blocking buffer, Enhanced BCA protein assay kit, SDS-PAGE gel kit, BeyoECL Plus kit, Annexin V-FITC cell apoptosis detection kit and PI cell cycle detection kit were purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: Cell Cycle Assay, Western Blot, Expressing, Incubation

Journal: Cell reports. Medicine

Article Title: Combined therapy with DR5-targeting antibody-drug conjugate and CDK inhibitors as a strategy for advanced colorectal cancer.

doi: 10.1016/j.xcrm.2025.102158

Figure Lengend Snippet: Figure 2. In vitro cytotoxicity of Oba01 in MSS and MSI-H CRC cell lines (A and B) DR5 expression in CRC cell lines assessed by western blotting (A) and immunofluorescence (B). Scale bars, 20 μm. (C) Cell viability after treatment with Oba01, zaptuzumab, MMAE, irinotecan, or oxaliplatin, measured by CellTiter-Glo Assay. (D–H) Cell survival upon treatment analyzed by cell confluency using the Incucyte S3 system. (I) Proliferation of Oba01-treated cells detected by EdU assay with confocal microscope. (J and K) Apoptosis of Oba01-treated cells assessed by the Annexin V-FITC and PI Apoptosis Kit. Scale bars, 20 μm. Data represent mean ± SEM of ≥3 experiments. p values from one-way ANOVA. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Cell apoptosis and cell cycle assay Cells were seeded at the density of 2 × 10 5 cells/well and exposed to Oba01 and/or Abem at the different concentrations for 48 h. Apoptosis was assessed using the Annexin V-FITC and PI Apoptosis Kit (Beyotime Biotechnology, Shanghai, China) and cell cycle status was analyzed by staining with the cell cycle assay kit (Beyotime Biotechnology, Shanghai, China).

Techniques: In Vitro, Expressing, Western Blot, Immunofluorescence, Glo Assay, EdU Assay, Microscopy